Description: Rabbit polyclonal antibody to TRIM27 Immunogen: Recombinant fusion protein of human TRIM27. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: TRIM27 Alternative Names: RFP; RNF76; Zinc finger protein RFP; RING finger protein 76; Ret finger protein; Tripartite motif-containing protein 27Entrez Gene (Human): 5987Entrez Gene (Mouse) : 19720SwissProt (Human): P14373SwissProt (Mouse) : Q62158Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TRIM27 expression in HepG2 (A), SHSY5Y (B) whole cell lysates.
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  Immunohistochemical analysis of TRIM27 staining in rat ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.