Description: Rabbit polyclonal antibody to TOP1MT Immunogen: Recombinant fusion protein of human TOP1MT. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: TOP1MT Alternative Names: DNA topoisomerase I mitochondrial; TOP1mtEntrez Gene (Human): 116447Entrez Gene (Rat) : 300029SwissProt (Human): Q969P6SwissProt (Rat) : Q6IM78Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TOP1MT expression in A549 (A) whole cell lysates.
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  Immunohistochemical analysis of TOP1MT staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.