Description: Rabbit polyclonal antibody to TMED3 Immunogen: Recombinant fusion protein of human TMED3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: TMED3 Alternative Names: C15orf22; Transmembrane emp24 domain-containing protein 3; Membrane protein p24B; p24 family protein gamma-4; p24gamma4; p26Entrez Gene (Human): 23423Entrez Gene (Mouse) : 66111Entrez Gene (Rat) : 300888SwissProt (Human): Q9Y3Q3SwissProt (Mouse) : Q78IS1SwissProt (Rat) : Q6AY25Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of TMED3 expression in A549 (A) whole cell lysates.
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  Immunohistochemical analysis of TMED3 staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of TMED3 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).