Description: Rabbit polyclonal antibody to TLR5 Immunogen: Recombinant fusion protein of human TLR5. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: TLR5 Alternative Names: TIL3; Toll-like receptor 5; Toll/interleukin-1 receptor-like protein 3Entrez Gene (Human): 7100SwissProt (Human): O60602Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TLR5 expression in HeLa (A) whole cell lysates.
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Immunohistochemical analysis of TLR5 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.