Description: Rabbit polyclonal antibody to TIAR Immunogen: Recombinant fusion protein of human TIAR. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: TIAL1 Alternative Names: Nucleolysin TIAR; TIA-1-related proteinEntrez Gene (Human): 7073Entrez Gene (Mouse) : 21843SwissProt (Human): Q01085SwissProt (Mouse) : P70318Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of TIAR expression in HeLa (A), K562 (B) whole cell lysates.
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Immunohistochemical analysis of TIAR staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.