Description: Rabbit polyclonal antibody to CKAP1 Immunogen: Recombinant fusion protein of human CKAP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: TBCB Alternative Names: CG22; CKAP1; Tubulin-folding cofactor B; Cytoskeleton-associated protein 1; Cytoskeleton-associated protein CKAPI; Tubulin-specific chaperone BEntrez Gene (Human): 1155Entrez Gene (Mouse) : 66411SwissProt (Human): Q99426SwissProt (Mouse) : Q9D1E6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CKAP1 expression in Jurkat (A), mouse brain (B), mouse heart (C) whole cell lysates.
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Immunohistochemical analysis of CKAP1 staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.