Description: Rabbit polyclonal antibody to SYCE1 Immunogen: Recombinant fusion protein of human SYCE1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: SYCE1 Alternative Names: C10orf94; Synaptonemal complex central element protein 1; Cancer/testis antigen 76; CT76Entrez Gene (Human): 93426Entrez Gene (Mouse) : 74075Entrez Gene (Rat) : 502372SwissProt (Human): Q8N0S2SwissProt (Mouse) : Q9D495SwissProt (Rat) : Q5XHZ2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SYCE1 expression in mouse liver (A), rat liver (B) whole cell lysates.
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Immunofluorescent analysis of SYCE1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.