Description: Rabbit polyclonal antibody to SC35 Immunogen: Recombinant fusion protein of human SC35. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: SRSF2 Alternative Names: SFRS2; Serine/arginine-rich splicing factor 2; Protein PR264; Splicing component 35 kDa; Splicing factor SC35; SC-35; Splicing factor arginine/serine-rich 2Entrez Gene (Human): 6427Entrez Gene (Mouse) : 20382Entrez Gene (Rat) : 494445SwissProt (Human): Q01130SwissProt (Mouse) : Q62093SwissProt (Rat) : Q6PDU1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SC35 expression in mouse lung (A) whole cell lysates.
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Immunohistochemical analysis of SC35 staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.