Description: Rabbit polyclonal antibody to SOD3 Immunogen: Recombinant fusion protein of human SOD3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: SOD3 Alternative Names: Extracellular superoxide dismutase [Cu-Zn]; EC-SODEntrez Gene (Human): 6649Entrez Gene (Mouse) : 20657Entrez Gene (Rat) : 25352SwissProt (Human): P08294SwissProt (Mouse) : O09164SwissProt (Rat) : Q08420Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SOD3 expression in HL60 (A), mouse kidney (B), mouse lung (C) whole cell lysates.
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Immunohistochemical analysis of SOD3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.