Description: Rabbit polyclonal antibody to N-SMase3 Immunogen: Recombinant fusion protein of human N-SMase3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: SMPD4 Alternative Names: KIAA1418; Sphingomyelin phosphodiesterase 4; Neutral sphingomyelinase 3; nSMase-3; nSMase3; Neutral sphingomyelinase IIIEntrez Gene (Human): 55627Entrez Gene (Mouse) : 77626SwissProt (Human): Q9NXE4SwissProt (Mouse) : Q6ZPR5Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of N-SMase3 expression in A549 (A) whole cell lysates.
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Immunofluorescent analysis of N-SMase3 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).