Description: Rabbit polyclonal antibody to SERT Immunogen: KLH-conjugated synthetic peptide of human SERT. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: SLC6A4 Alternative Names: HTT; SERT; Sodium-dependent serotonin transporter; 5HT transporter; 5HTT; Solute carrier family 6 member 4Entrez Gene (Human): 6532Entrez Gene (Mouse) : 15567Entrez Gene (Rat) : 25553SwissProt (Human): P31645SwissProt (Mouse) : Q60857SwissProt (Rat) : P31652Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of SERT expression in U251MG (A), mouse lung (B) whole cell lysates.
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  Immunohistochemical analysis of SERT staining in rat testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.