Description: Rabbit polyclonal antibody to SLC39A8 Immunogen: Recombinant fusion protein of human SLC39A8. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: SLC39A8 Alternative Names: BIGM103; ZIP8; Zinc transporter ZIP8; BCG-induced integral membrane protein in monocyte clone 103 protein; LIV-1 subfamily of ZIP zinc transporter 6; LZT-Hs6; Solute carrier family 39 member 8; Zrt- and Irt-like protein 8; ZIP-8Entrez Gene (Human): 64116Entrez Gene (Mouse) : 67547SwissProt (Human): Q9C0K1SwissProt (Mouse) : Q91W10Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SLC39A8 expression in HeLa (A), mouse liver (B) whole cell lysates.
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Immunofluorescent analysis of SLC39A8 staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Western blot analysis of SLC39A8 expression in HL60 (A) whole cell lysates. (Predicted band size: 43; 48; 49 kD; Observed band size: 50 kD)