Description: Rabbit polyclonal antibody to SKAP2 Immunogen: Recombinant fusion protein of human SKAP2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: SKAP2 Alternative Names: PRAP; RA70; SAPS; SCAP2; SKAP55R; Src kinase-associated phosphoprotein 2; Pyk2/RAFTK-associated protein; Retinoic acid-induced protein 70; SKAP55 homolog; SKAP-55HOM; SKAP-HOM; Src family-associated phosphoprotein 2; Src kinase-associated phosphoprotein 55-related protein; Src-associated adapter protein with PH and SH3 domainsEntrez Gene (Human): 8935Entrez Gene (Mouse) : 54353Entrez Gene (Rat) : 155183SwissProt (Human): O75563SwissProt (Mouse) : Q3UND0SwissProt (Rat) : Q920G0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SKAP2 expression in mouse lung (A) whole cell lysates.
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Immunohistochemical analysis of SKAP2 staining in mouse stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of SKAP2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.