Description: Rabbit polyclonal antibody to SFRP1 Immunogen: Recombinant fusion protein of human SFRP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: SFRP1 Alternative Names: FRP; FRP1; SARP2; Secreted frizzled-related protein 1; FRP-1; sFRP-1; Secreted apoptosis-related protein 2; SARP-2Entrez Gene (Human): 6422Entrez Gene (Mouse) : 20377SwissProt (Human): Q8N474SwissProt (Mouse) : Q8C4U3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of SFRP1 expression in HEK293T (A), mouse kidney (B) whole cell lysates.
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Immunohistochemical analysis of SFRP1 staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.