Description: Rabbit polyclonal antibody to Alpha-NaCH Immunogen: Recombinant fusion protein of human Alpha-NaCH. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: SCNN1A Alternative Names: SCNN1; Amiloride-sensitive sodium channel subunit alpha; Alpha-NaCH; Epithelial Na(+) channel subunit alpha; Alpha-ENaC; ENaCA; Nonvoltage-gated sodium channel 1 subunit alpha; SCNEAEntrez Gene (Human): 6337Entrez Gene (Mouse) : 20276Entrez Gene (Rat) : 25122SwissProt (Human): P37088SwissProt (Mouse) : Q61180SwissProt (Rat) : P37089Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Alpha-NaCH expression in mouse lung (A) whole cell lysates.
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  Immunofluorescent analysis of Alpha-NaCH staining in human lung cancer. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).