Description: Rabbit polyclonal antibody to RSPO1 Immunogen: Recombinant fusion protein of human RSPO1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RSPO1 Alternative Names: R-spondin-1; Roof plate-specific spondin-1; hRspo1Entrez Gene (Human): 284654Entrez Gene (Mouse) : 192199SwissProt (Human): Q2MKA7SwissProt (Mouse) : Q9Z132Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RSPO1 expression in Jurkat (A) whole cell lysates.
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Immunohistochemical analysis of RSPO1 staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.