Description: Rabbit polyclonal antibody to Rag A Immunogen: Recombinant fusion protein of human Rag A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RRAGA Alternative Names: Ras-related GTP-binding protein A; Rag A; RagA; Adenovirus E3 14.7 kDa-interacting protein 1; FIP-1Entrez Gene (Human): 10670Entrez Gene (Mouse) : 68441Entrez Gene (Rat) : 117044SwissProt (Human): Q7L523SwissProt (Mouse) : Q80X95SwissProt (Rat) : Q63486Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Rag A expression in HeLa (A), mouse spleen (B), rat brain (C) whole cell lysates.
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Immunohistochemical analysis of Rag A staining in human placenta formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.