Description: Rabbit polyclonal antibody to Ribosomal Protein L32 Immunogen: Recombinant fusion protein of human Ribosomal Protein L32. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: RPL32 Alternative Names: 60S ribosomal protein L32Entrez Gene (Human): 6161Entrez Gene (Mouse) : 102642959;19951Entrez Gene (Rat) : 28298SwissProt (Human): P62910SwissProt (Mouse) : P62911SwissProt (Rat) : P62912Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Ribosomal Protein L32 expression in HepG2 (A), NIH3T3 (B), mouse spleen (C), rat ovary (D) whole cell lysates.
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Immunohistochemical analysis of Ribosomal Protein L32 staining in rat brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of Ribosomal Protein L32 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.