Description: Rabbit polyclonal antibody to RBM15 Immunogen: Recombinant fusion protein of human RBM15. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RBM15 Alternative Names: OTT; OTT1; Putative RNA-binding protein 15; One-twenty two protein 1; RNA-binding motif protein 15Entrez Gene (Human): 64783SwissProt (Human): Q96T37Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of RBM15 expression in HepG2 (A), Jurkat (B), mouse spleen (C) whole cell lysates.
-
Immunohistochemical analysis of RBM15 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.