Description: Rabbit polyclonal antibody to RAPsyn Immunogen: Recombinant fusion protein of human RAPsyn. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RAPSN Alternative Names: RNF205; 43 kDa receptor-associated protein of the synapse; RAPsyn; 43 kDa postsynaptic protein; Acetylcholine receptor-associated 43 kDa protein; RING finger protein 205Entrez Gene (Human): 5913Entrez Gene (Mouse) : 19400SwissProt (Human): Q13702SwissProt (Mouse) : P12672Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RAPsyn expression in B cells (A), mouse skeletal muscle (B), rat brain (C) whole cell lysates.
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Immunohistochemical analysis of RAPsyn staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.