Description: Rabbit polyclonal antibody to RABGAP1 Immunogen: Recombinant fusion protein of human RABGAP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: RABGAP1 Alternative Names: Rab GTPase-activating protein 1; GAP and centrosome-associated protein; Rab6 GTPase-activating protein GAPCenAEntrez Gene (Human): 23637Entrez Gene (Mouse) : 227800SwissProt (Human): Q9Y3P9SwissProt (Mouse) : A2AWA9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of RABGAP1 expression in HeLa (A), mouse brain (B) whole cell lysates.
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Immunohistochemical analysis of RABGAP1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.