Description: Rabbit polyclonal antibody to PSMB3 Immunogen: Recombinant fusion protein of human PSMB3. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: PSMB3 Alternative Names: Proteasome subunit beta type-3; Proteasome chain 13; Proteasome component C10-II; Proteasome theta chainEntrez Gene (Human): 5691Entrez Gene (Mouse) : 26446Entrez Gene (Rat) : 29676SwissProt (Human): P49720SwissProt (Mouse) : Q9R1P1SwissProt (Rat) : P40112Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PSMB3 expression in NIH3T3 (A), MCF7 (B), mouse brain (C), rat liver (D) whole cell lysates.
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Immunohistochemical analysis of PSMB3 staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.