Description: Rabbit polyclonal antibody to PRPF31 Immunogen: Recombinant fusion protein of human PRPF31. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: PRPF31 Alternative Names: PRP31; U4/U6 small nuclear ribonucleoprotein Prp31; Pre-mRNA-processing factor 31; Serologically defined breast cancer antigen NY-BR-99; U4/U6 snRNP 61 kDa protein; Protein 61K; hPrp31Entrez Gene (Human): 26121Entrez Gene (Mouse) : 68988SwissProt (Human): Q8WWY3SwissProt (Mouse) : Q8CCF0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PRPF31 expression in Jurkat (A), mouse testis (B), rat lung (C) whole cell lysates.
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Immunohistochemical analysis of PRPF31 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.