Description: Rabbit polyclonal antibody to PRAMEF20/21 Immunogen: Recombinant fusion protein of human PRAMEF20/21. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: PRAMEF20; PRAMEF21 Alternative Names: PRAME family member 20/21Entrez Gene (Human): 391001;645425Entrez Gene (Mouse) : 627009SwissProt (Human): Q5VT98Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PRAMEF20/21 expression in mouse heart (A), rat heart (B) whole cell lysates.
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  Immunohistochemical analysis of PRAMEF20/21 staining in mouse brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.