Description: Rabbit polyclonal antibody to I-plastin Immunogen: Recombinant fusion protein of human I-plastin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: PLS1 Alternative Names: Plastin-1; Intestine-specific plastin; I-plastinEntrez Gene (Human): 5357Entrez Gene (Mouse) : 102502SwissProt (Human): Q14651SwissProt (Mouse) : Q3V0K9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of I-plastin expression in HEK293T (A), mouse brain (B), mouse liver (C), rat spleen (D) whole cell lysates.
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  Immunohistochemical analysis of I-plastin staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.