Description: Rabbit polyclonal antibody to PIP5K1-beta Immunogen: Recombinant fusion protein of human PIP5K1-beta. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: PIP5K1B Alternative Names: STM7; Phosphatidylinositol 4-phosphate 5-kinase type-1 beta; PIP5K1-beta; PtdIns(4)P-5-kinase 1 beta; Phosphatidylinositol 4-phosphate 5-kinase type I beta; PIP5KIbeta; Protein STM-7; Type I phosphatidylinositol 4-phosphate 5-kinase betaEntrez Gene (Human): 8395Entrez Gene (Mouse) : 18719Entrez Gene (Rat) : 309419SwissProt (Human): O14986SwissProt (Mouse) : P70181SwissProt (Rat) : Q5CZZ9Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of PIP5K1-beta expression in mouse lung (A) whole cell lysates.
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Immunofluorescent analysis of PIP5K1-beta staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.