Description: Rabbit polyclonal antibody to PIG-M Immunogen: Recombinant fusion protein of human PIG-M. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: PIGM Alternative Names: GPI mannosyltransferase 1; GPI mannosyltransferase I; GPI-MT-I; Phosphatidylinositol-glycan biosynthesis class M protein; PIG-MEntrez Gene (Human): 93183Entrez Gene (Mouse) : 67556Entrez Gene (Rat) : 79112SwissProt (Human): Q9H3S5SwissProt (Mouse) : Q8C2R7SwissProt (Rat) : Q9EQY6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of PIG-M expression in BxPC3 (A), mouse spleen (B), mouse lung (C), rat spleen (D) whole cell lysates.
-
Immunohistochemical analysis of PIG-M staining in rat kidney formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
-
Immunofluorescent analysis of PIG-M staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).