Description: Rabbit polyclonal antibody to PI 4-kinase beta Immunogen: Recombinant fusion protein of human PI 4-kinase beta. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: PI4KB Alternative Names: PIK4CB; Phosphatidylinositol 4-kinase beta; PI4K-beta; PI4Kbeta; PtdIns 4-kinase beta; NPIK; PI4K92Entrez Gene (Human): 5298Entrez Gene (Mouse) : 107650Entrez Gene (Rat) : 81747SwissProt (Human): Q9UBF8SwissProt (Mouse) : Q8BKC8SwissProt (Rat) : O08561Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of PI 4-kinase beta expression in mouse liver (A), rat brain (B), rat lung (C) whole cell lysates.
-
Immunofluorescent analysis of PI 4-kinase beta staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).