Description: Rabbit polyclonal antibody to PHF11 Immunogen: Recombinant fusion protein of human PHF11. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: PHF11 Alternative Names: BCAP; PHD finger protein 11; BRCA1 C-terminus-associated protein; Renal carcinoma antigen NY-REN-34Entrez Gene (Human): 51131Entrez Gene (Mouse) : 219132Entrez Gene (Rat) : 361051SwissProt (Human): Q9UIL8SwissProt (Mouse) : A6H5X4SwissProt (Rat) : Q5I0E2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of PHF11 expression in SW480 (A) whole cell lysates.
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  Immunohistochemical analysis of PHF11 staining in mouse lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of PHF11 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.