Description: Rabbit polyclonal antibody to Podoplanin Immunogen: Recombinant fusion protein of human Podoplanin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: PDPN Alternative Names: GP36; Podoplanin; Aggrus; Glycoprotein 36; Gp36; PA2.26 antigen; T1-alpha; T1AEntrez Gene (Human): 10630Entrez Gene (Mouse) : 14726Entrez Gene (Rat) : 54320SwissProt (Human): Q86YL7SwissProt (Mouse) : Q62011SwissProt (Rat) : Q64294Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Podoplanin expression in mouse brain (A), rat kidney (B) whole cell lysates.
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  Immunohistochemical analysis of Podoplanin staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.