Description: Rabbit polyclonal antibody to NUP50 Immunogen: Recombinant fusion protein of human NUP50. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NUP50 Alternative Names: NPAP60L; Nuclear pore complex protein Nup50; 50 kDa nucleoporin; Nuclear pore-associated protein 60 kDa-like; Nucleoporin Nup50Entrez Gene (Human): 10762Entrez Gene (Mouse) : 18141Entrez Gene (Rat) : 25497SwissProt (Human): Q9UKX7SwissProt (Mouse) : Q9JIH2SwissProt (Rat) : O08587Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of NUP50 expression in HeLa (A), mouse thymus (B) whole cell lysates.
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  Immunohistochemical analysis of NUP50 staining in rat testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.