Description: Rabbit polyclonal antibody to NUBP1 Immunogen: Recombinant fusion protein of human NUBP1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: NUBP1 Alternative Names: NBP; NBP1; Cytosolic Fe-S cluster assembly factor NUBP1; Nucleotide-binding protein 1; NBP 1Entrez Gene (Human): 4682SwissProt (Human): P53384Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NUBP1 expression in HepG2 (A) whole cell lysates.
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Immunohistochemical analysis of NUBP1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.