Description: Rabbit polyclonal antibody to NFE2L1 Immunogen: Recombinant fusion protein of human NFE2L1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: NFE2L1 Alternative Names: HBZ17; NRF1; TCF11; Nuclear factor erythroid 2-related factor 1; NF-E2-related factor 1; NFE2-related factor 1; Locus control region-factor 1; Nuclear factor erythroid derived 2 like 1; Transcription factor 11; TCF-11; Transcription factor HBZ17; Transcription factor LCR-F1Entrez Gene (Human): 4779Entrez Gene (Mouse) : 18023SwissProt (Human): Q14494SwissProt (Mouse) : Q61985Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of NFE2L1 expression in Jurkat (A), PC12 (B), mouse lung (C) whole cell lysates.
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Immunohistochemical analysis of NFE2L1 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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Immunofluorescent analysis of NFE2L1 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.