Description: Rabbit polyclonal antibody to Nicastrin Immunogen: Recombinant fusion protein of human Nicastrin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: NCSTN Alternative Names: KIAA0253; NicastrinEntrez Gene (Human): 23385Entrez Gene (Mouse) : 59287Entrez Gene (Rat) : 289231SwissProt (Human): Q92542SwissProt (Mouse) : P57716SwissProt (Rat) : Q8CGU6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Nicastrin expression in C6 (A), mouse brain (B), rat brain (C) whole cell lysates.
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Immunofluorescent analysis of Nicastrin staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).