Description: Rabbit polyclonal antibody to Mesothelin Immunogen: Recombinant fusion protein of human Mesothelin. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/200) Gene Symbol: MSLN Alternative Names: MPF; Mesothelin; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factorEntrez Gene (Human): 10232SwissProt (Human): Q13421Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Mesothelin expression in HEK293T (A) whole cell lysates.
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Immunohistochemical analysis of Mesothelin staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.