Description: Rabbit polyclonal antibody to Multimerin-1 Immunogen: Recombinant fusion protein of human Multimerin-1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IF/IC (1/50 - 1/200) Gene Symbol: MMRN1 Alternative Names: ECM; EMILIN4; GPIA; MMRN; Multimerin-1; EMILIN-4; Elastin microfibril interface located protein 4; Elastin microfibril interfacer 4; Endothelial cell multimerinEntrez Gene (Human): 22915Entrez Gene (Mouse) : 70945SwissProt (Human): Q13201SwissProt (Mouse) : B2RPV6Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Multimerin-1 expression in HepG2 (A), mouse spleen (B) whole cell lysates.
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Immunofluorescent analysis of Multimerin-1 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.