Description: Rabbit polyclonal antibody to MED25 Immunogen: Recombinant fusion protein of human MED25. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: MED25 Alternative Names: ACID1; ARC92; PTOV2; Mediator of RNA polymerase II transcription subunit 25; Activator interaction domain-containing protein 1; Activator-recruited cofactor 92 kDa component; ARC92; Mediator complex subunit 25; p78Entrez Gene (Human): 81857Entrez Gene (Mouse) : 75613SwissProt (Human): Q71SY5SwissProt (Mouse) : Q8VCB2Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MED25 expression in A549 (A), mouse brain (B), rat kidney (C) whole cell lysates.
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Immunofluorescent analysis of MED25 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.