Description: Rabbit polyclonal antibody to ARMET Immunogen: Recombinant fusion protein of human ARMET. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: MANF Alternative Names: ARMET; ARP; Mesencephalic astrocyte-derived neurotrophic factor; Arginine-rich protein; Protein ARMETEntrez Gene (Human): 7873SwissProt (Human): P55145Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ARMET expression in MCF7 (A) whole cell lysates.
-
Immunofluorescent analysis of ARMET staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 488-conjugated secondary antibody (green) in PBS at room temperature in the dark.