Description: Rabbit polyclonal antibody to MANBA Immunogen: Recombinant fusion protein of human MANBA. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: MANBA Alternative Names: MANB1; Beta-mannosidase; Lysosomal beta A mannosidase; Mannanase; MannaseEntrez Gene (Human): 4126Entrez Gene (Mouse) : 110173SwissProt (Human): O00462SwissProt (Mouse) : Q8K2I4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of MANBA expression in mouse testis (A) whole cell lysates.
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Immunohistochemical analysis of MANBA staining in human liver cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.