Description: Rabbit polyclonal antibody to Lunatic Fringe Immunogen: Recombinant fusion protein of human Lunatic Fringe. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: LFNG Alternative Names: Beta-1 3-N-acetylglucosaminyltransferase lunatic fringe; O-fucosylpeptide 3-beta-N-acetylglucosaminyltransferaseEntrez Gene (Human): 3955Entrez Gene (Mouse) : 16848Entrez Gene (Rat) : 170905SwissProt (Human): Q8NES3SwissProt (Mouse) : O09010SwissProt (Rat) : Q924T4Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of Lunatic Fringe expression in PC12 (A), HeLa (B), mouse lung (C) whole cell lysates.
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  Immunohistochemical analysis of Lunatic Fringe staining in human stomach formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.