Description: Rabbit polyclonal antibody to KLF8 Immunogen: Recombinant fusion protein of human KLF8. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: KLF8 Alternative Names: BKLF3; ZNF741; Krueppel-like factor 8; Basic krueppel-like factor 3; Zinc finger protein 741Entrez Gene (Human): 11279Entrez Gene (Mouse) : 245671SwissProt (Human): O95600SwissProt (Mouse) : Q8BLM0Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of KLF8 expression in HeLa (A), mouse brain (B), rat brain (C) whole cell lysates.
-
Immunofluorescent analysis of KLF8 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).