Description: Rabbit polyclonal antibody to KIF22 Immunogen: Recombinant fusion protein of human KIF22. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: KIF22 Alternative Names: KID; KNSL4; Kinesin-like protein KIF22; Kinesin-like DNA-binding protein; Kinesin-like protein 4Entrez Gene (Human): 3835Entrez Gene (Mouse) : 110033SwissProt (Human): Q14807SwissProt (Mouse) : Q3V300Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of KIF22 expression in A549 (A), HeLa (B), mouse testis (C) whole cell lysates.
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Immunohistochemical analysis of KIF22 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.