Description: Rabbit polyclonal antibody to JMJD1A Immunogen: KLH-conjugated synthetic peptide of human JMJD1A. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200), IF/IC (1/50 - 1/200) Gene Symbol: KDM3A Alternative Names: JHDM2A; JMJD1; JMJD1A; KIAA0742; TSGA; Lysine-specific demethylase 3A; JmjC domain-containing histone demethylation protein 2A; Jumonji domain-containing protein 1AEntrez Gene (Human): 55818Entrez Gene (Mouse) : 104263Entrez Gene (Rat) : 312440SwissProt (Human): Q9Y4C1SwissProt (Mouse) : Q6PCM1SwissProt (Rat) : Q63679Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of JMJD1A expression in HEK293T (A) whole cell lysates.
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  Immunohistochemical analysis of JMJD1A staining in mouse testis formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
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  Immunofluorescent analysis of JMJD1A staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.