Description: Rabbit polyclonal antibody to INSIG2 Immunogen: KLH-conjugated synthetic peptide of human INSIG2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/1000), IH (1/50 - 1/100) Gene Symbol: INSIG2 Alternative Names: Insulin-induced gene 2 protein; INSIG-2Entrez Gene (Human): 51141Entrez Gene (Mouse) : 72999SwissProt (Human): Q9Y5U4SwissProt (Mouse) : Q91WG1Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of INSIG2 expression in HEK293T (A), mouse brain (B) whole cell lysates.
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  Immunohistochemical analysis of INSIG2 staining in rat ovary formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.