Description: Rabbit polyclonal antibody to CD210b Immunogen: Recombinant fusion protein of human CD210b. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IF/IC (1/50 - 1/200) Gene Symbol: IL10RB Alternative Names: CRFB4; D21S58; D21S66; Interleukin-10 receptor subunit beta; IL-10 receptor subunit beta; IL-10R subunit beta; IL-10RB; Cytokine receptor class-II member 4; Cytokine receptor family 2 member 4; CRF2-4; Interleukin-10 receptor subunit 2; IL-10R subunit 2; IL-10R2; CDw210bEntrez Gene (Human): 3588SwissProt (Human): Q08334SwissProt (Mouse) : Q61190Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of CD210b expression in Jurkat (A), mouse skeletal muscle (B), rat liver (C) whole cell lysates.
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Immunofluorescent analysis of CD210b staining in L929 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).