Description: Rabbit polyclonal antibody to IEX1 Immunogen: KLH-conjugated synthetic peptide of human IEX1. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: IER3 Alternative Names: DIF2; IEX1; PRG1; Radiation-inducible immediate-early gene IEX-1; Differentiation-dependent gene 2 protein; Protein DIF-2; Immediate early protein GLY96; Immediate early response 3 protein; PACAP-responsive gene 1 protein; Protein PRG1Entrez Gene (Human): 8870SwissProt (Human): P46695SwissProt (Mouse) : P46694Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of IEX1 expression in HeLa (A), A431 (B) whole cell lysates.
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Immunohistochemical analysis of IEX1 staining in human gastric cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.