Description: Rabbit polyclonal antibody to ICA69 Immunogen: Recombinant fusion protein of human ICA69. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: ICA1 Alternative Names: Islet cell autoantigen 1; 69 kDa islet cell autoantigen; ICA69; Islet cell autoantigen p69; ICAp69; p69Entrez Gene (Human): 3382SwissProt (Human): Q05084Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
-
Western blot analysis of ICA69 expression in U87MG (A) whole cell lysates.
-
Immunohistochemical analysis of ICA69 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.