Description: Rabbit polyclonal antibody to Connexin 47 Immunogen: KLH-conjugated synthetic peptide of human Connexin 47. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: GJC2 Alternative Names: GJA12; Gap junction gamma-2 protein; Connexin-46.6; Cx46.6; Connexin-47; Cx47; Gap junction alpha-12 proteinEntrez Gene (Human): 57165Entrez Gene (Mouse) : 118454Entrez Gene (Rat) : 497913SwissProt (Human): Q5T442SwissProt (Mouse) : Q8BQU6SwissProt (Rat) : Q80XF7Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of Connexin 47 expression in mouse brain (A) whole cell lysates.
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Immunohistochemical analysis of Connexin 47 staining in human kidney cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.