Description: Rabbit polyclonal antibody to GCC2 Immunogen: Recombinant fusion protein of human GCC2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: GCC2 Alternative Names: KIAA0336; RANBP2L4; GRIP and coiled-coil domain-containing protein 2; 185 kDa Golgi coiled-coil protein; GCC185; CLL-associated antigen KW-11; CTCL tumor antigen se1-1; Ran-binding protein 2-like 4; RanBP2L4; Renal carcinoma antigen NY-REN-53Entrez Gene (Human): 9648SwissProt (Human): Q8IWJ2SwissProt (Mouse) : Q8CHG3Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.
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Western blot analysis of GCC2 expression in Raji (A), HEK293T (B) whole cell lysates.
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Immunohistochemical analysis of GCC2 staining in human colon formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.