Description: Rabbit polyclonal antibody to HNF-3 alpha Immunogen: Recombinant fusion protein of human HNF-3 alpha. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography. Clonality: Polyclonal Form: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. Dilution: WB (1/500 - 1/2000), IH (1/50 - 1/200) Gene Symbol: FOXA1 Alternative Names: HNF3A; TCF3A; Hepatocyte nuclear factor 3-alpha; HNF-3-alpha; HNF-3A; Forkhead box protein A1; Transcription factor 3A; TCF-3AEntrez Gene (Human): 3169Entrez Gene (Mouse) : 15375Entrez Gene (Rat) : 25098SwissProt (Human): P55317SwissProt (Mouse) : P35582SwissProt (Rat) : P23512Storage/Stability : Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

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  Western blot analysis of HNF-3 alpha expression in MCF7 (A), HeLa (B), mouse lung (C) whole cell lysates.
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  Immunohistochemical analysis of HNF-3 alpha staining in rat lung formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.